Table II.

Metabolism of recombinant OsGA2ox1 protein

Substrate	Producta	KRI	Characteristic Ions at m/z (% Relative Intensity of Base Peak)b
2H2-GA44	GA98	2,962	522 (47), 507 (5), 373 (4), 345 (4), 240 (21), 209 (100)
2H2-GA20	GA29	2,688	509 (100), 494 (6), 450 (2), 379 (6), 210 (25)
2H3-GA1	GA8	2,818	597 (100), 582 (7), 538 (8), 451 (5), 210 (20)
2H3-GA9	GA51	2,539	389 (22), 331 (30), 287 (78), 271 (76), 227 (100)
GA4	GA34	2,678	506 (100), 459 (1), 416 (3), 313 (7), 288 (3)

Substrate GAs were incubated with recombinant OsGA2ox1 protein, and the product GAs were identified by full-scan GC-MS and KRI.

^a Identification of metabolic products by GC-MS on the basis of KRI and full-scan mass spectra of the methyl ester trimethylsilylether derivatives. 

^b Based on ions with a mass-to-charge ratio (m/z) of >200.