Figure 3.

HCV E1E2 folding determines the efficiency of association with viral and cellular proteins. ^A4HAHCVcc-infected cells were lysed with Triton X-100 (Tx) buffer. The resulting cell lysate was subjected to immunoprecipitation with anti-E2 (AR3A), anti-E1E2 (AR4A), and anti-E1E2 (AR5A) antibodies, and with isotypic IgG. The immunoprecipitated proteins were subjected to immunoblotting with anti-E2, anti-E1, anti-NS3, and anti-HA antibodies. Protein bands were quantified by densitometry using ImageJ software. AR3A immunoprecipitation band relative density is 100. The western blots presented here are representative of multiple experiments (≥3) showing similar results.