Figure 4.

TNS4 was directly controlled by miR-150-3p in LUAD cells. (A,B) TNS4 mRNA and protein expression was reduced by miR-150-3p ectopic expression (48 h after transfection). GUSB was used as an expression control. GAPDH was used as a loading control. * p < 0.01. (C) Dual luciferase reporter assays using vectors encoding the wild-type TNS4 3′-UTR sequence containing one putative miR-150-3p target site (wild) and 3′-UTR sequences with deletions of the target site (deletion). Normalized data were calculated as the ratio of Renilla/firefly luciferase activities. * p < 0.01. UTR: untranslated region.