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. 2019 Apr 30;11(5):601. doi: 10.3390/cancers11050601

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of TNS4 overexpression by H1299 cells. (A) Expression levels of TNS4 protein 48 h after transfection with pCMV-TNS4 vector into H1299 cells. (B) Cell proliferation was evaluated by XTT assays. (C) Cell migration was determined by wound healing assay 24 h after forward transfection with the pCMV-TNS4 vector. (D) Cell invasion was determined by Matrigel assay 24 h after forward transfection with the pCMV-TNS4 vector. These assays showed that migratory and invasive activities were increased in pCMV-TNS4 vector-transfected cell lines. * p < 0.01.