Figure 5.

Effect of pre-adipocyte and adipocyte conditioned media (ACM) on endometrial cancer stem cell activity and response to metformin. (a) Schematic of adipocyte maturation and conditioned media generation and images at ×100 magnification of pre-adipocytes and mature adipocytes stained with Oil Red O after 10, 12 and 15 days of exposure to adipocyte differentiation media. (b) SRB cytotoxicity assay of Ishikawa cells exposed to ACM and treated with metformin (n = 4). (c) SFE of Ishikawa cells treated with individual patient adipocyte conditioned media (ACM, n = 6). (d) SFE of Ishikawa cells exposed to ACM and metformin (n = 6). Data are represented as mean ± SEM from individual patient ACM. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.