Table 2.
Antibodies used in this study.
| Primary Antibodies | |||||
| Target | Company | Number | Species | IHC | Western Blot |
| AFP | Dako | A0008 | Rabbit | 1:250 | - |
| β-Actin | Merck | A5441 | Mouse | - | 1:25000 |
| EOMES | Abcam | ab23345 | Rabbit | 1:200 | - |
| FOXA2 | R&D systems | AF2400 | Goat | 1:200 | 1:500 |
| Ki67 | Zytomed Systems | MSK018 | Mouse | 1:500 | - |
| OCT3/4 | Santa Cruz | C-10 | Mouse | 1:200 | - |
| PRAME | Santa Cruz | H-10 | Mouse | - | 1:400 |
| PRDM1 | H.M. Jäck | - | Rabbit | 1:200 | - |
| SOX2 | R&D systems | MAB2018 | Mouse | 1:200 | 1:200 |
| SOX17 | Abcam | ab84990 | Mouse | 1:400 | - |
| SOX17 | R&D systems | AF1924 | Goat | - | 1:1000 |
| TFAP2C | Santa Cruz | 6E4/4 | Mouse | 1:200 | - |
| Secondary Antibodies | |||||
| Target | Company | Number | Species | IHC | Western Blot |
| Rabbit Anti-Goat HRP | Dako | P0160 | Rabbit | - | 1:2000 |
| Rabbit Anti-Mouse HRP | Dako | P0260 | Rabbit | - | 1:1000 |