Figure 7.
Effect of pinocembrin concentration in insulin glycation kinetics. Insulin samples were incubated at 37 °C with 0.5 M d-ribose (InsRib) or 0.5 mM methylglyoxal (InsMG) at different concentrations of pinocembrin (0, 0.1, 1, 10, and 20 mM) and AGE fluorescence (λex 320 nm/λem 410 nm) was monitored after 7 days of incubation. Ins: Insulin incubated in the absence of glycating agent. Other experimental details are described in Section 2. Data are means ± SE (n = 5). *** p ˂ 0.001 versus InsRib, ### p ˂ 0.001 versus InsMG.
