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Glycated insulin–pinocembrin interaction monitored by fluorescence spectroscopy. Tyrosine fluorescence emission was evaluated on in native insulin (Ins) and in insulin glycated by d-ribose (InsRib) and methylglyoxal (InsMG) in the presence and in the absence of pinocembrin. Working concentrations were 10 µM for insulin and 10 µM for pinocembrin. Other experimental details are described in Section 2.
