Figure 3.

Knockdown of EBP1 inhibited the suppressive effect of YC-1 on HCC cells. HCC cells were transfected with scrambled and EBP1-specific shRNA #2 for 24 h and recovered for 48 h. Scrambled shRNA-transfected and EBP1-silenced cells were treated with the IC₅₀ of YC-1 for 6 h. Luciferase activity was measured by STF reporter assays (A). The indicated cells were seeded in 6-well plates at 5 × 10⁴ cells per well and incubated for 48 h. After the HCC cells were treated with the IC₅₀ of YC-1 for 24 h, the medium was replaced. The colonies formed from the indicated cells were observed and counted (B). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: p > 0.05.