Figure 1.

Sh3bp2 gain-of-function mutation improves the survival rate of Fas^lpr lupus-prone mice. (a) Immunoblot analysis for SH3BP2. Protein samples were collected from lymph nodes and spleens of WT, Sh3bp2^KI/+, Fas^lpr/lpr, and Sh3bp2^KI/+Fas^lpr/lpr mice. Actin was used as a loading control. (b–d) WT (n = 8), Sh3bp2^KI/+ (n = 7), Fas^lpr/lpr (n = 12), and Sh3bp2^KI/+Fas^lpr/lpr mice (n = 8) were monitored until the age of 48 weeks. At the end of the observation period, body weight (b) and spleen weight (c) were measured. (d) Survival rates of the mice. Values are presented as the mean ± SD. Note: * p < 0.05; n.s. = not significant. SH3BP2, SH3 domain-binding protein 2; WT, wild-type; KI, knock-in.