Figure 2.

Sh3bp2 gain-of-function mutation improves renal involvement in Fas^lpr lupus-prone mice. (a) Incidence of proteinuria. WT (n = 7), Sh3bp2^KI/+ (n = 7), Fas^lpr/lpr (n = 5), and Sh3bp2^KI/+Fas^lpr/lpr mice (n = 6) were monitored until the age of 48 weeks, and levels of proteinuria were monitored every 8 weeks and graded as follows: 0, <30 mg/dL; 1+, 30–99 mg/dL; 2+, 100–299 mg/dL; 3+, >300 mg/dL. Incidence of proteinuria (≥2+) is presented. (b) Images of periodic acid-Schiff (PAS)-stained kidney sections from 48-week-old mice. Original magnification, 400×. Bar, 10 μm. (c) Renal glomerulosclerosis score. Severity of glomerulosclerosis was graded from 0 to 4, with 15 to 20 glomeruli graded per mouse. Values are presented as the mean ± SD. Note: * p < 0.05. SH3BP2, SH3 domain-binding protein 2; WT, wild-type; KI, knock-in