Figure 3.

Sh3bp2 gain-of-function mutation suppresses aberrant anti-dsDNA antibody and immunoglobulin production in Fas^lpr lupus-prone mice. (a,b) Serum samples were collected from WT (n = 7), Sh3bp2^KI/+ (n = 7), Fas^lpr/lpr (n = 5), and Sh3bp2^KI/+Fas^lpr/lpr mice (n = 5) at 16, 32, and 48 weeks of age. Levels of anti-dsDNA antibody (a) and each immunoglobulin subclass (b) were determined by ELISA. Values are presented as the mean ± SD. * p < 0.05; n.s. = not significant. Note: dsDNA, double-stranded DNA; SH3BP2, SH3 domain-binding protein 2; WT, wild-type; KI, knock-in; ELISA, enzyme-linked immunosorbent assay.