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. 2019 Apr 30;8(5):402. doi: 10.3390/cells8050402

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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B cell differentiation and maturation are unaltered in Sh3bp2^KI/+Fas^lpr/lpr mice. (a–c) B cell subsets were analyzed in the bone marrow (a) and spleen (b,c) by flow cytometry. Cells were collected from WT (n = 7), Sh3bp2^KI/+ (n = 7), Fas^lpr/lpr (n = 5), and Sh3bp2^KI/+Fas^lpr/lpr mice (n = 6) at 48 weeks of age, and the suspended cells were stained with fluorochrome-labeled antibodies against IgM, IgD, CD43, and B220 for bone marrow cells (a) and IgM, IgD, CD19, CD23, and CD21/35 for splenic cells (b,c). All cells were initially gated as 7-AAD-negative single cells, followed by being gated as lymphocytes (a,b) or CD19⁺ cells (c). Values are presented as the mean ± SD. Note: * p < 0.05; n.s. = not significant. SH3BP2, SH3 domain-binding protein 2; WT, wild-type; KI, knock-in; 7-AAD, 7-aminoactinomycin D.