Table 1.
The quantitative PCR (qPCR) primers used in this study.
| Target Genes | Gene Accession Number | Sequence (5′-3′) | Amplicon Size (bp) | |
|---|---|---|---|---|
| Fas | NM_007987.2 | Forward: Reverse: |
taaaccagacttctactgcgattct gggttccatgttcacacga |
73 |
| Fasl | NM_010177.4 | Forward: Reverse: |
aaaaagagccgaggagtgtg attccagagggatggacctt |
66 |
| Tnf | NM_013693.2 | Forward: Reverse: |
catcttctcaaaattcgagtgaca tgggagtagacaaggtacaaccc |
175 |
| Tnfr1 | NM_011609.4 | Forward: Reverse: |
ggaaagtatgtccattctaagaacaa agtcactcaccaagtaggttcctt |
76 |
| Tnfr2 | NM_011610.3 | Forward: Reverse: |
gaggcccaagggtttcag ggcttccgtgggaagaat |
64 |
| Trail | NM_ 009425.2 | Forward: Reverse: |
ggctgcaagtctgcattggg cctgcagctgcttcatctcgt |
192 |
| Trailr2 | NM_ 020275.4 | Forward: Reverse: |
ccacaacacggaacctggca tgtctgtgaggcccaactgc |
167 |
| Tweak | NM_ 011614.3 | Forward: Reverse: |
caggatggagcacaagcag ggctggagctgttgattttg |
73 |
| Dr3 | NM_ 001291010.1 | Forward: Reverse: |
ccctggcttatcccagact agatgccagaggagttccaa |
97 |
| Hprt | NM_013556.2 | Forward: Reverse: |
tcctcctcagaccgctttt cctggttcatcatcgctaatc |
90 |
Fasl, Fas ligand; Tnf, tumor necrosis factor; Tnfr1, TNF receptor type 1; Tnfr2, TNF receptor type 2; Trail, TNF-related apoptosis-inducing ligand; Trailr2, TRAIL receptor 2; Tweak, TNF-like weak inducer of apoptosis; Dr3, death receptor 3; Hprt, hypoxanthine phosphoribosyltransferase.