. 2019 May 2;11(5):616. doi: 10.3390/cancers11050616

Table 2.

Applications of CSC enrichment methods in HNSCC.

Enrichment Only from Cell Culture	Enrichment from Surgically Excised or Primary Cultured Patient Material	Enrichment from Blood Samples
Low proteasome activity (ZsGreen-cODC reporter) [26,56]	Cell surface proteins (antibody-based methods) [20]	Cell surface proteins (antibody-based methods) [58]
Low proteasome activity (ZsGreen-cODC reporter) [26,56]	Aldehyde dehydrogenase (ALDH) activity (Aldefluor assay) [25]
Stem cell transcription factors (fluorescent reporters) [42]	Sphere formation assay [55]
	Side population (Hoechst 33342 exclusion assay) [57]