Figure 3.

Renieramycin T (RT) induced activation of PARP, caspase-3, and caspase-9 in the H460 cell line. Moreover, RT also significantly up-regulated p53 and down-regulated Mcl-1 in the H460 cell line. H460 cells were treated with RT (0–25 µM) for 24 h. (A) and (C) Apoptotis-related proteins were measured with Western blot analysis. The blots were reprobed with β-actin to confirm equal loading of each of the protein samples. (B) and (D) The relative protein levels were calculated by densitometry. Data represented the mean ± SEM (n = 3) (* 0.01 ≤ p < 0.05, ** p < 0.01, compared with the untreated control).