Figure 4.

HSF1 interacted with the 5′-flanking regions of SELENOF. (a) PCR amplification results of chromatin immunoprecipitation (ChIP) primers after protein-chromatin cross-linkage and immunoprecipitation. Locations of JASPAR predicted HSF1 binding sites and the designed ChIP primers were presented in the schematic map. (b) Manually searched results of both typical and less conserved variant of HSF1 Sequence-binding Elements (HSEs) in the sequences of SELENOF 5′- flanking region from −1430 to −248. The data are representative of three independent experiments.