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. 2019 May 18;8(5):479. doi: 10.3390/cells8050479

Figure 5.

Figure 5

Heat shock treatment increased SELENOF transcriptional regulation. (a) Luciferase reporter activities of SELENOF core positive regulatory element in transfected HEK293T cells with/without heat shock treatment. Transcriptional levels of SELENOF (b) and heat shock protein (HSP)70 (c) at several time intervals in HEK293T cells under heat shock treatment. (d) Protein levels of SELENOF in control and HSF1 over-expressed HEK293T cells with/without heat shock treatment. The data are representative of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001.