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. 2019 May 22;11(5):705. doi: 10.3390/cancers11050705

Figure 1.

Figure 1

Integrin α11 regulates lysyl oxidase-like1 (LOXL1) mRNA expression. (a) Integrin α11 (ITGA11) and LOXL1 gene expression are strongly correlated in three independent non-small cell lung cancer (NSCLC) datasets. Spearman correlation between ITGA11 and LOXL1 gene expression was performed using RNA Seq data of The Cancer Genome Atlas (TCGA) lung adenocarcinoma dataset (n = 517), gene expression microarray dataset from University Health Network (UHN, n = 128, GSE50081) and Sungkyunkwan University (SKKU, n = 63, GSE8894). (b) ITGA11 and LOXL1 mRNA expression was analyzed using RT-qPCR and showed expression in cancer-associated fibroblasts (CAFs, n = 20) but not in lung normal fibroblasts (NFs, n = 9) or in established NSCLC cell lines (n = 30). CAF094 that has been used in further experiments is highlighted in green (c). LOXL1 expression depends on ITGA11 expression. LOXL1 expression has been studied by RT-qPCR (left panel) in wild-type mouse embryonic fibroblast (MEF) (MEF α11+/+), integrin α11 knockout MEF (MEF α11−/−), and integrin α11 knock-in MEF (MEF α11−/− KI) and (right panel) in wild-type C2C12 cells and C2C12 cells expressing integrin α11 (C2C12 α11) (n = 3 per condition, **, p < 0.01; ***, p < 0.001; mean ± SD).