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. 2019 May 14;10(5):368. doi: 10.3390/genes10050368

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of AON-mediated exon 36 skipping on CEP290 mRNAs. (A) RT-PCR analysis of CEP290 transcripts expressed in control (C3) and patient (P1 and P2) fibroblasts untreated or treated for 24h with lipofectamine alone or associated to H36D AON. Images of agarose gel showing amplicons produced using primer pairs surrounding mutant exon 36. (B) Relative expression levels of full-length (black bars) and exon 36-skipped (grey bars) CEP290 mRNAs in control (C1–C3) and patient (P1 and P2) fibroblasts as determined by RT-qPCR. Bars show the mean ± SEM from three independent experiments. C represents the pooled values of C1–C3. **** p ≤ 0.0001, n.s., not significant.