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Effect of menthol treatment on: (A) Relative expression of energy expenditure genes in 3T3-L1 cells at 1 μM, 10 μM, 30 µM and 50 μM menthol treatment; (B)Relative expression of energy expenditure genes in 3T3-L1 cells at 1 μM menthol treatment for 1 h. n = 3–5; All the values are expressed as mean ± S.E.M; Analysis was carried out by student paired t-test and one-way ANOVA followed by Tukey’s multiple comparison; * p < 0.05, ** p < 0.01, *** p < 0.001.
