Table 1.
Assembly metrics denoted for each of the Red Sea brine pool and other hydrothermal vent samples.
| Description | Detailed Description | Sites | Reference Sampling & Read Sequences/Assembly | Number of Reads | Number of Reads after Trimming | Number of Assembled Reads | Number of Contigs > 500 bp | Average Contig Size (bp) | Largest Contig Size (bp) |
|---|---|---|---|---|---|---|---|---|---|
| Atlantis II water column (Water above brine pool) | Atlantis II 50 m water column | ATII 50 | [19]/This study | 1,461,910 | 1,461,904 | 582,768 | 36,262 | 1149 | 21,887 |
| Atlantis II 200 m water column | ATII 200 | 1,260,578 | 1,260,561 | 530,441 | 34,640 | 1131 | 25,392 | ||
| Atlantis II 700 m water column | ATII 700 | 1,128,514 | 1,128,507 | 554,335 | 32,860 | 1285 | 33,783 | ||
| Atlantis II 1500 m water column | ATII 1500 | 833,739 | 833,730 | 316,101 | 20,374 | 1331 | 51,927 | ||
| Atlantis II brine water | Atantis II brine-seawater interface | ATII INF | [20]/This study | 832,138 | 832,128 | 743,064 | 9933 | 1214 | 25,150 |
| Atantis II brine Upper Convective Layer | ATII UCL | 886,030 | 886,019 | 794,715 | 11,994 | 1454 | 103,389 | ||
| Atantis II brine Lower Convective Layer | ATII LCL | 4,104,966 | 4,104,994 | 3,901,967 | 19,165 | 2084 | 350,936 | ||
| Discovery Deep brine water | Discovery Deep brine-seawater interface | DD INF | [20]/This study | 1,095,181 | 1,095,157 | 752,025 | 14,144 | 1201 | 28,080 |
| Discovery Deep brine water | DD BR | 1,111,044 | 1,111,032 | 763,387 | 15,306 | 1216 | 22,118 | ||
| Kebrit Deep brine water | Kebrit Deep Upper brine-seawater interface | KD UINF | [20]/This study | 1,562,521 | 1,562,512 | 1,020,749 | 24,517 | 1495 | 58,542 |
| Kebrit Deep Lower brine-seawater interface | KD LINF | 1,510,272 | 1,510,262 | 926,337 | 31,983 | 1241 | 38,825 | ||
| Kebrit Deep brine water | KD BR | 1,379,832 | 1,379,814 | 913,803 | 22,280 | 945 | 14,864 | ||
| Other water metagenomes | Guaymas Basin deep-sea hydrothermal vent plume water | GB VNT | [39,40,41]/This study | 628,619 | 628,569 | 155,841 | 7654 | 1082 | 17,353 |
| Kueishantao shallow-sea hydrothermal vent (water above vent) | KSW VNT | [42]/This study | 261,446 | 261,399 | 199,237 | 411 | 4685 | 179,360 | |
| Kueishantao shallow-sea hydrothermal vent (water) | K VNT | [42]/This study | 444,655 | 444,597 | 338,480 | 2194 | 1843 | 88,498 | |
| Juan de Fuca Ridge hydrothermal vent diffuse flow seawater | JDF VNT | [43]/This study | 226,981 | 226,916 | 35,357 | 9366 | 1135 | 9366 | |
| Sediments | Atlantis II sediments | ATII SDM | [28,36]/This study | 1,138,406 | 1,138,381 | 478,453 | 30,352 | 1194 | 33,674 |
| Discovery Deep sediments | DD SDM | 1,258,290 | 1,258,273 | 597,552 | 38,529 | 1233 | 38,081 | ||
| Non-brine sediments | NB SDM | 253,568 | 253,564 | 92,530 | 7292 | 1177 | 1315 | ||
| Other metagenome (biofilm) | Loki’s Castle deep-sea vent biofilm (microbial mat) | LC MM | [44]/This study | 717,550 | 717,135 | 525,719 | 7897 | 1546 | 42,387 |
| Total | 22,096,240 | 22,095,454 | 14,222,861 | 377,153 | - | - |