Table 2.
SMGCs abundance and the counts of archaeal and bacterial phyla in each site. SMGCs are named using the same acronyms as those used by by antiSMASH tool [45]. Hyphens indicate hybrid clusters. Cf_fatty_acid: fatty acid putative cluster. Cf_saccharide: saccharide putative cluster. Hserlactone: cluster coding for homoserine lactone. NRPS: cluster coding for non-ribosomal peptide synthetase. OtherKS: cluster coding for other types of polyketide synthases. Pufa: cluster coding for poly-unsaturated fatty acids. T1pks: type I polyketide synthase. T2pks: type II polyketide synthase. T3pks: type III polyketide synthase. Acyl_amino_acids: cluster coding for N-acyl amino acid.
| Detailed Description | Assembly | Number of SMGCs | Normalized Number of SMGCs * | Types of SMGCs | Number of Phyla | SMGCs Detected Uniquely Once at a Particular Site |
|---|---|---|---|---|---|---|
| Red Sea metagenomic samples: | ||||||
| Atlantis II 1500 m water column | ATII 1500 | 168 | 531.48 | 9 | 33 | Otherks-Pufa-T1pks, T2pks-Cf_fatty_acid |
| Atlantis II 700 m water column | ATII 700 | 269 | 485.27 | 8 | 32 | Otherks-Pufa, Otherks-T1pks |
| Kebrit Deep Lower brine-seawater interface | KD LINF | 334 | 360.56 | 9 | 33 | Cf_saccharide-Bacteriocin, Hserlactone |
| Atlantis II 200 m water column | ATII 200 | 170 | 320.49 | 8 | 30 | Cf_fatty_acid-Arylpolyene |
| Atantis II brine Upper Convective Layer | ATII UCL | 210 | 264.25 | 7 | 21 | |
| Atlantis II 50 m water column | ATII 50 | 146 | 250.53 | 8 | 30 | |
| Kebrit Deep Upper brine-seawater interface | KD UINF | 252 | 246.88 | 13 | 32 | Ladderane-Cf_fatty_acid, Nrps-T1pks, T1PKS |
| Atantis II brine-seawater interface | ATII INF | 162 | 218.02 | 6 | 19 | |
| Discovery Deep sediments | DD SDM | 114 | 190.78 | 4 | 23 | |
| Non-brine sediments | NB SDM | 16 | 172.92 | 4 | 9 | |
| Kebrit Deep brine water | KD BR | 149 | 163.05 | 4 | 23 | |
| Atlantis II sediments | ATII SDM | 70 | 146.30 | 7 | 26 | |
| Atantis II brine Lower Convective Layer | ATII LCL | 524 | 134.29 | 13 | 25 | Cf_fatty_acid-Cf_saccharide, Cf_saccharide-nrps, Phosphonate, T3pks-cf_saccharide |
| Discovery Deep brine-seawater interface | DD INF | 94 | 125.00 | 1 | 20 | |
| Discovery Deep brine water | DD BR | 73 | 95.63 | 2 | 20 | |
| Other metagenomic samples: | ||||||
| Guaymas Basin deep-sea hydrothermal vent plume water | GB VNT | 11 | 70.58 | 3 | 30 | Pufa |
| Kueishantao shallow-sea hydrothermal vent (water above vent) | KSW VNT | 11 | 55.21 | 4 | 12 | Thiopeptide |
| Kueishantao shallow-sea hydrothermal vent (water) | K VNT | 10 | 29.54 | 5 | 13 | |
| Juan de Fuca Ridge hydrothermal vent diffuse flow seawater | JDF VNT | 3 | 84.85 | 3 | 14 | |
| Loki’s Castle deep-sea vent biofilm (microbial mat) | LC MM | 26 | 49.46 | 5 | 23 | Acyl_amino_acids |
* Normalized number of SMGCs are the number of SMGCs detected at each site divided by the number of assembled reads *106.