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. 2019 May 13;11(5):662. doi: 10.3390/cancers11050662

Figure 1.

Figure 1

The induction of mesothelial-mesenchymal transition (MMT) in the peritoneal mesothelial cells (PMCs) exposed to autologous and cancer-derived conditioned medium (CM). (a) Representative pictures showing the development of spindle-shaped morphologies in the PMCs and confirmed by changes in E-cadherin (green) and vimentin (red) expression (the nuclei are stained blue). The very left panel of pictures shows the identification of PMCs according to the expression of HBME-1 antigen (brown cytoplasm). Below, HBME-1-negative endothelial cells (HMEC-1). Scale bars = 50 µm. (b) Changes in the E-cadherin and vimentin concentration in the cell lysates from the PMCs exposed to autologous and cancer-derived CM. (c) Migration of the PMCs undergoing the cancer-induced MMT. Experiments were performed with eight separate cultures of PMCs obtained from different donors, with eight separate cultures of primary epithelial cancer cells (pEOC), and with eight separate cultures of each established cancer cell line. The results are expressed as the mean ± SEM. *, p < 0.05 vs. PMCs; **, p < 0.01 vs. PMCs; ***, p < 0.001 vs. PMCs; #, p < 0.05 vs. A2780; Ψ, p < 0.05 vs. SKOV-3. RFU, relative fluorescence units.