Table I.
Genetic analysis of slr1 and slr2
| Genetic Treatment | WT | Mutant | Observed Segregation | Expected Segregation | χ2 |
|---|---|---|---|---|---|
| Segregation of slr1 | |||||
| Initial F2 population | 12 | 3 | 4.0:1 | 3:1 | 0.20 |
| Selfed heterozygous WT (7)a | 90 | 22 | 4.1:1 | 3:1 | 1.72 |
| Selfed homozygous WT (8) | 121 | 0 | – | – | – |
| Outcross of slr1 (A632 × slr1) | |||||
| F2 from the cross (3) | 108 | 13 | 8.3:1b | 3:1 | 32.28 |
| Outcross of slr1 (B73 × slr1) | |||||
| F2 from the cross (6) | 156 | 19 | 8.2:1b | 3:1 | 18.67 |
| Segregation of slr2 | |||||
| Initial F2 population | 8 | 7 | 1.1:1 | 3:1 | 3.76 |
| Selfed heterozygous WT (7) | 196 | 74 | 2.6:1 | 3:1 | 0.84 |
| Selfed homozygous WT (7) | 166 | 0 | – | – | – |
| Selfed mutant (5) | 2 | 117 | – | – | – |
| Outcross of slr2 (slr2 × SWS2000) | |||||
| F2 from the cross (13) | 586 | 183 | 3.2:1 | 3:1 | 0.60 |
a
No. of analyzed plants in parentheses.
b
These segregation ratios might be indicative for 15:1 as a result of statistic analysis in A632 (χ2 = 4.0941, p = 0.01) and B73 (χ2 = 6.4192, p = 0.01) genetic backgrounds.