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. 2019 May 13;10(5):367. doi: 10.3390/genes10050367

Figure 1.

Figure 1

Multiple alignment and transmembrane region prediction of ETMP30 and its Entamoeba homologs. (a) Multiple alignment of ETMP30 and its Entamoeba orthologs was built using MAFFT [29]. The alignment was displayed using Jalview [30]. The hydrophobic, positively charged, negatively charged, hydrophilic, glycine, and proline residues are colored green, blue, red, light blue, yellow, and orange, respectively. Boxes and dashed boxes show predicted transmembrane region of ETMP30 and the region having relatively lower ΔG, respectively. (b) Prediction of transmembrane regions of ETMP30 and its ortholog using ΔG prediction for transmembrane insertion [21]. Gray boxes and light gray dashed boxes show predicted transmembrane region of ETMP30 and the region having relatively lower ΔG, respectively.