Figure 6.

Effects of H₂O₂ on Prx6 multimers in human cells. (A) Human pulmonary artery smooth muscle cells were pre-treated with or without 50 μM deferoxamine for 30 min and then treated with or without H₂O₂ for 15 min. Cell lysates were prepared and subjected to SDS-PAGE with or without 5% (v/v) BME in Laemmli buffer, and immunoblotted with the Prx6 antibody. No Protein-SHifters were added in these experiments. (B–D) Graphs represent the means ± SEM of the intensity of the 50-kDa band (n = 3–6). The symbol * represents the value significantly different from the untreated control value at p < 0.05.