Figure 8.

Effects of H₂O₂ on the isolated proteins. (A) Cell lysates were prepared from untreated human pulmonary artery smooth muscle cells (Lane 2) or cells treated with H₂O₂ for 15 min (Lane 1). Lysate proteins from untreated cells were denatured with SDS and incubated with H₂O₂ (Lane 3) or BME (Lane 4) in a test tube for 15 min. Protein-SHifter Plus was then added and the samples subjected to SDS-PAGE and immunoblotted with the Prx6 antibody. The bar graph represents means ± SEM of the intensity of the 50-kDa band (n = 3). The symbol * represents the value significantly different from the untreated control value at p < 0.05. (B) The purified recombinant CNBD domain of the hHCN4 channel was treated with H₂O₂ (1 mM) for 15 min and subjected to SDS-PAGE followed by Coomassie Blue staining. No Protein-SHifters were used in these experiments.