Figure 4.

PB125 prevented oxidative stress-induced loss of cell viability. Cytotoxicity was not observed (cell proliferation measured by CCK8 assay) in HepG2 cells treated overnight with 5 μg/mL PB125 compared to vehicle control blank. In HepG2 cells cultured for 18h with 5 μg/mL PB125 or vehicle control, from which the culture media was removed and replaced and the cells were challenged with an oxidative stress by treatment with 25 μM cumene hydroperoxide (CHP) or untreated control for 6 h, cell toxicity (loss of viability) was caused by cumene hydroperoxide treatment but this toxicity was partially attenuated (p < 0.05) by PB125 pretreatment. ns: not significant.