Figure 5.

PB125 prevented oxidative stress-induced cell injury. Cellular injury (release of LDH into the culture media) was not observed in HepG2 cells treated overnight with 5 μg/mL PB125 compared to vehicle control blank. In HepG2 cells cultured for 18h with 5 μg/mL PB125 or vehicle control, from which the culture media was removed and replaced and the cells were challenged with an oxidative stress by treatment with 25 μM cumene hydroperoxide (CHP) or untreated control for 6 h, cell injury (release of LDH) was caused by cumene hydroperoxide treatment but this injury was partially attenuated (p < 0.05) by PB125 pretreatment.