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. 2019 May 8;8(5):424. doi: 10.3390/cells8050424

Figure 1.

Figure 1

Shh is overexpressed in PDAC in the epithelial and stromal compartments: (a) SHH gene expression in tumour tissues compared to paired normal tissues (* p < 0.05 Wilcoxon matched-pairs signed rank test). (b) Quantification of IHC staining (H-score) for Shh protein expression in normal and tumour tissues (* p < 0.05 Wilcoxon matched-pairs signed rank test). (c) Example images of Shh IHC staining in normal pancreatic tissue (NT), and moderately differentiated pancreatic tumour (MDT), and poorly differentiated pancreatic tumour (PDT) tissues. Negative controls demonstrate the absence of staining either in normal tissue (column on the left) or PDT tissue (column on the right) when an isotype control antibody was substituted for the specific antibody to the target. Red arrows show epithelial Shh expression and black arrows show stromal Shh expression. Semi-quantitative analysis of the percentage Shh staining in the epithelial (d) and stromal (e) compartments (* p = < 0.05 Kruskal-Wallis multiple comparison Test) for NT, MDT, or PDT samples. Analysis of correlation (Spearman correlation test) between % vimentin staining and % epithelial (* p = 0.015) (f) or stromal (* p = 0.04) (g) Shh staining.