Figure 5.

kai2, max2, d14 mutants support a near‐normal response to mechano‐stimulus.

Karrikin (KAR)‐ and strigolactone (SL)‐insensitive mutants showed a normal upregulation of touch response genes, in response to mechanical stimulation. Nine‐day‐old seedlings of wild‐type and mutants (a) kai2‐1 and kai2‐2, and (b) d14, max2‐1 were mechanically stimulated (MS) for 30 sec, then collected 30 min later for transcript analysis of touch‐sensitive genes CML12 and CML24, relative to housekeeping genes Tubulin 4 and Ubiquitin 10. The means of six–nine replicates from three independent experiments are shown, each replicate based on the RNA extracted from roots of 30–40 seedlings. Data are shown as mean ± se, letters indicate significant differences (Tukey HSD, P < 0.01).

(c) Mechano‐stimulated [Ca²⁺]_cyt increase in max2 root tips. Individual excised root tips of Col‐0 and max2 expressing (apo)aequorin as a [Ca²⁺]_cyt reporter were mechanically stimulated by addition of buffer at 35 sec. The mean ± SEM of 40–67 roots in five independent trials are shown. Inset: mean ± SEM maximal [Ca²⁺]_cyt increment in response to stimulus (peak response minus baseline).