Figure 2.

Light microscopy image (×10 magnification) of third passage bone marrow derived MSCs at day seven (a), compared third passage day seven peripheral blood MSCs mobilized with VEGF and AMD3100 (b). Bone marrow MSCs were obtained from the femoral shaft of rats of a similar age and isolated by plastic adherence. Peripheral blood MSCs were obtained by cardiac puncture 60 min after a single dose of AMD3100, preceded by a 4 day course of VEGF, once daily, every 24 h. The cells were isolated by plastic adherence after lysing the red blood cells.