Figure 3.

In vitro assessment of anti-tumor activity of chemokine receptor-expressing CAR T-cells targeted against αvβ6. Pancreatic (A), triple negative breast (B), or ovarian (C) tumor cells were co-cultivated at a 1:1 ratio with the indicated CAR T-cells in the absence of exogenous cytokine for 24–72 h. Data show the mean ± SEM of residual tumor cell viability from 3 independent experiments, each performed in triplicate and quantified by MTT assay. At each time-point, percentage tumor cell survival has been expressed relative to untreated tumor cells (set at 100% viability). Supernatant was collected and levels of IFN-γ (48 h; D) and IL-2 (24 h; E) quantified by ELISA. Data show the mean ± SEM of cytokine concentration detected in 3 independent experiments.