Figure 5.

Effect of loss of RNG2 on Ca²⁺‐ and cGMP‐induced microneme protein (MIC) and granule protein (GRA) secretion. (a–c) Extracellular secretion assays (ESA) with and without ATc‐induced RNG2 depletion in iΔHA‐RNG2 cells. Depletion of HARNG2 is seen by immuno‐detection of HA in the cell pellet. A23187 (5μM) is used for Ca²⁺ stimulation (i), and BIPPO (2.5μM) is used for cGMP stimulation (ii). Relative MIC2 (b) and GRA1 (c) is shown for eight (Ca²⁺, b(i), c(i)) and 10 (cGMP, b(ii), c(ii)) biological replicates. TOM40 in ESA serves a control for cell lysis and controls for cell‐equivalents loading in the pellet. Error bars = SEM