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. 2019 May 16;11(5):684. doi: 10.3390/cancers11050684

Figure 7.

Figure 7

Cyclin E knockdown inhibits virus replication only in MCF7/pS cells. (A) Cells were either left uninfected (Ctrl) or infected with AdGFP or OAdmCherry at a multiplicity of infection (MOI) concentration of five alone or in combination with 500 nM palbociclib for 24 h. Cyclin E and viral E1A expression was assessed by immunoblotting. (B) Cells were transfected with either control or a pool of cyclin E-specific siRNAs followed by AdGFP or OAdmCherry at a MOI concentration of five alone or in combination with 500 nM palbociclib for 24 h. Cyclin E expression and downstream signaling as well as expression of E1A viral protein was assessed by immunoblotting. Quantitative densitometry analysis is shown relative to loading control normalized to palbociclib-sensitive control-treated cells (except for E1A blots where samples were normalized to OAdmCherry Ctrl). (C) mCherry expression was evaluated by fluorescence microscopy. Scale: 200 µm. (D) Cell number was assessed by crystal violet staining and calculated by measuring the absorbance of solubilized dye at 590 nm. Results are expressed as percent (%) of AdGFP vehicle-treated cells. Error bars, ±SEM of three independent experiments. * p < 0.05; *** p < 0.001; compared to control AdGFP unless otherwise indicated.