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. 2019 May 3;11(5):414. doi: 10.3390/v11050414

Figure 1.

Figure 1

Transcription activator-like effector nuclease (TALEN) targeting and cleavage on the target site. The specific repeat variable di-residue (RVD) used to recognize each base is indicated by shading, as defined in the key (NI: A, NG: T, NN: G, HD: C). The cleavage site is in the adjoining region between the left and right target site. The left transcription activator-like effector (TALE) and the right TALE of TALEN plasmids recognized their target sequence and allowed their associated Fok I endonucleases to work as homodimers to cleave the sense strand 9 bp and antisense strand 13 bp downstream of the binding site. The binding of the TALEs to the target sites allows Fok I to dimerize and create a double-stranded break (DSB) with sticky ends within the spacer.