Figure 6.

(A) Viral DNA targeting and cleavage: Once TALENs target and cleave the viral DNA, they will create a double stranded break (DSB). Either repaired by homologous recombination (HR) or non-homologous end joining (NHEJ), a mild mutation such as mismatch may appear. If viral DNA is mutated seriously or not repaired, viruses will be dead (highlighted in green). Viruses without cleavage, viruses without mutations, and viruses only with mild mutations are all alive (highlighted in yellow). (B) Surveyor nuclease mutation detection assay: Total DNA of all alive viruses are harvested and amplified by the polymerase chain reaction (PCR), followed by the denaturation, hybridization and analysis of the PCR products. Surveyor nuclease is a mismatch-specific endonuclease.