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. 2019 May 3;11(5):414. doi: 10.3390/v11050414

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TALEN plasmid transfection was prior to MCMV infection: (A) Only homoduplex (or heteroduplex) PCR products (1048 bp) were observed (Lane 2–7). (B) Only MCMV3-4 (Lane 4) target and cleave PCR products produced two extra DNA bands (608 and 440 bp) clearly. MCMV1-2 and 5-6 (Lane 3 and 5) could not produce dominant extra bands, compared with the negative control (Lane 2). Neither the nonspecific TALEN plasmids W1FS-W7R1 (Lane 6) nor KSHV1-2 (Lane 7) could produce any extra bands, the same as the negative control (Lane 2). MCMV infection was prior to TALEN plasmid transfection: (C) Only homoduplex (or heteroduplex) PCR products (1048 bp) were observed (Lane 2-7). (D) No extra bands produced by TALEN plasmids were observed (Lane 3–7). Only positive control G + C (Lane 8) could produce dominant extra bands (416 and 217 bp) in addition to a major band (633 bp), compared with the negative control (Lane 2).