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. 2019 May 17;11(5):453. doi: 10.3390/v11050453

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of RNA-seq results by quantitative real-time PCR (RT-qPCR). Expression patterns of 12 representative genes as determined by RT-qPCR and RNA-seq. Normalization for RT-qPCR was performed using expression of the actin gene as an internal reference. CAHC: Carbonic anhydrase, chloroplastic; PAE8: Pectin acetylesterase 8; PLY5: Pectate lyase 5; LECR: Lectin-related protein; DCL2: Endoribonuclease Dicer homolog 2; SDE3: Silencing defective 3; MGL: Methionine gamma-lyase; PCNA: Proliferating cell nuclear antigen; GSTX2: Glutathione S-transferase; SAG12: Senescence-specific cysteine protease SAG12; PR4B: Pathogenesis-related protein PR-4B; GID1B: Gibberellin receptor GID1B.