Table 1.
Validation parameters of the method.
| Analytes | Matrix | Spiking Level (ng/mL) | Recovery (%) | RSD c (%) | LOD *d | LOQ *e |
|---|---|---|---|---|---|---|
| DON a | Grains (malt) | 100 | 92.1 | 9.2 | 3.5 | 11.6 |
| 500 | 92.9 | 2.9 | ||||
| 1000 | 87.6 | 4.4 | ||||
| Liquid (wort) | 50 | 90.6 | 1.4 | 0.5 | 1.6 | |
| 100 | 91.5 | 2.0 | ||||
| 250 | 94.6 | 1.4 | ||||
| ZEN b | Grains (malt) | 50 | 89.6 | 5.3 | 2.8 | 9.2 |
| 250 | 99.8 | 2.9 | ||||
| 500 | 105.7 | 0.4 | ||||
| Liquid (wort) | 10 | 85.8 | 1.1 | 2.0 | 6.7 | |
| 50 | 89.2 | 0.9 | ||||
| 100 | 97.9 | 0.8 |
* grain: µg/kg; liquid: µg/L; a DON: deoxynivalenol; b ZEN: zearalenone; c RSD: relative standard deviation; d LOD: limit of detection; e LOQ: limit of quantification.