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. 2019 May 22;11(5):466. doi: 10.3390/v11050466

Figure 3.

Figure 3

Amentoflavone inhibits infection by ACV-resistant HSV-1 strains. (A) Vero cells were infected with HSV-1/106, HSV-1/153, and HSV-1/Blue (MOI = 0.1) in the presence of amentoflavone or ACV for 72 h. The antiviral effect was assessed by CCK8 assay. (B) Vero cells were infected with HSV-1/106, HSV-1/153, and HSV-1/Blue (MOI = 0.1) for 2 h. The viruses were then removed and cover fluid with amentoflavone was added. After 72 h, the cells were fixed and strained with crystal violet dye. The plaque numbers were counted to calculated the inhibitory effect. Data are mean ± SD (n = 3). ** p < 0.01, *** p < 0.001 versus HSV-1-treated group.