Figure 8.

Amentoflavone inhibits the promoter activity of viral immediate early gene. (A) Vero cells were infected with HSV-1 (MOI = 1) in the presence of amentoflavone (50 μM) or DMSO for 3 h and the expression of ICP0 and ICP4 was detected by qRT-PCR, respectively. Data are mean ± SD (n = 3). ** p < 0.01; *** p < 0.001. (B) Vero cells seeded in 48-well plates were transfected with indicated plasmid combinations for 24 h, the cell lysates were then subjected to luciferase activity assays. The cells only transfected with pGL-α0 plasmids (pGL-α4 plasmids) or cotransfected with pGL-α0 and pcDNA plasmids (pGL-α4 and pcDNA plasmids) were treated as the negative control. Data are mean ± SD (n = 3). * p < 0.05.