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. 2019 May 11;11(5):266. doi: 10.3390/toxins11050266

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Venom samples contain multiple proteins and peptides which differ in intensity over time with bleaching. Ten µg of protein from the reconstituted venom samples was loaded onto a 12% SDS-PAGE, two biological replicates (Anemone 1 and Anemone 2) from week 0, 9, and 18 were compared. (a) BioRad stain-free fluorescence protocol which highlights tryptophan containing peptides and proteins; and (b) BioRad Coomassie Brilliant Blue R-250 staining. Bands of interest are circled in red and labelled a–d.