Figure 1.

(a) Organization of the fusaoctaxin A gene cluster (fgm1-5 + 9) in F. graminearum and activation of key enzymes by in situ overexpression of NRPS5 and ex situ overexpression of NRPS9. The yellow ovals show positions of inserted constitutive promoters. (b) Extracted ion chromatograms of the protonated ions [M+H]⁺ for fusapentaxin A (red), fusatrixin A (green), fusatetraxin A (purple) and fusaoctaxin A (black) in F. graminearum wild type and overexpression strains. (c) Structures of the different fusaoctaxin A’s with chemical formula and monoisotopic mass (MM).