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. 2019 May 1;11(5):409. doi: 10.3390/v11050409

Figure 6.

Figure 6

Viral replication is not needed for NF-κB inhibition. (A,B) S2* cells were treated with 20-hydroxyecdysone (EcD) as indicated for 18 h and then infected with IIV-6 (blue circles) or uninfected (black circles) for six hours. Cells were then stimulated with DAP-type PGN for six hours, where indicated. Diptericin levels were monitored by qRT-PCR. (A) Cells were treated with cidofovir, a viral polymerase inhibitor, where indicated. Mock cells untreated with EcD, PGN, or cidofovir are shown as a control. Each data point is a biologically independent replicate; n = 3. Error bars represent standard deviation. Statistics were determined using two-way ANOVA and Sidak’s multiple comparisons test; ns, not significant; ****, p < 0.0001. (B) S2* cells were infected with IIV-6 (blue circles), or treated with UV- (purple circles) or heat- (red circles) inactivated IIV-6. Uninfected controls are shown in black. Each data point is a biologically independent replicate; n = 3. Error bars represent standard deviation. Statistics were determined using one-way ANOVA and Tukey’s multiple comparisons test; ns, not significant; ***, p < 0.001.