Flies infected with IIV-6 have lower AMP levels. (A) Adult w1118 flies were infected with IIV-6, injected with PBS, or left unmanipulated for 7 days, and then snap frozen for RNA isolation. Dpt levels were assayed by qRT-PCR. Black bars indicate mean and error bars indicate standard deviation. Statistics were determined using one-way ANOVA and Tukey’s multiple comparisons test; ns, not significant; *, p = 0.0463; **, p = 0.0033. (B) Kaplan-Meier plots of adult w1118 flies infected with IIV-6 (blue lines) or PBS-injected (black lines) for 8 days prior to bacterial infection. On day 0, flies were pricked with Ecc15 (solid lines) or were sterile pricked (dashed lines) with a microsurgery needle. Flies were counted daily for survivors. Statistics were determined using log-rank test; ****, p < 0.0001.