Figure 5.

Protease activity is a general property of PLA₂ enzymes: (A) Quantification of casein substrate degraded by bee venom PLA₂, bovine pancreatic PLA₂ and AAV2. The y- and x-axes are as described in Figure 4. (B) Predicted structure of AAV2 VP1u compared to bee venom PLA₂ (PDB ID: 1POC), bovine pancreatic PLA₂ (PDB ID: 1UNE) and human sPLA₂ (PDB ID: 1KQU). (C) Partial amino acid sequence alignment of PLA₂ enzymes (conserved residues are highlighted in red). Statistical significance as indicated by asterisk annotations (*) are described in Material and Methods section.