Figure 12.

The mRNA expression level of apoptosis gene by ZEA (50 µM), OTA (50 µM) and their enzymatic degradation final products. (A) Caspase3, (B) Sirt1, (C) Bax, (D) IL6, (E) TNFα, (F) Bcl2 mRNA expression level in the Lx2 cells. Cells were exposed to ZEA/OTA and enzymatic degradation derivatives for 24 h. The results are expressed relative to the expression of β-Actin; each set of data shows the mean ± SD of the three independent experiments. *, **, *** indicates a significant difference between ZEA, OTA and control at p < 0.05, p < 0.01, and p < 0.001. ns: not significant. #, ##, ###, indicates a significance difference between ZEA/OTA compare with their enzymatic final products at p < 0.05, p < 0.01, and p < 0.001.