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. 2019 May 4;11(5):416. doi: 10.3390/v11050416

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western blot analysis for sample of testing interaction between RGV-63R and RGV-91R by co-IP. Cell lysates from HEK293T cells cotransfected with indicated plasmids (91R-HA+ pcDNA3.1, 91R-HA+ 63R-3Flag) and IP (immunoprecipitated) protein complexes with indicated plasmids (91R-HA+ pcDNA3.1, 91R-HA+ 63R-3Flag) are subjected to Western blot analysis using anti-HA and anti-Flag. Cells lysates and IP showed the bands of 91R-HA and 63R-3Flag. M: protein molecular mass marker.